RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia.

ABSTRACT:

Background

Aging is affected by genetic and environmental factors, and cigarette smoking is strongly associated with accumulation of senescent cells. In this study, we wanted to identify genes that may potentially be beneficial for cell survival in response to cigarette smoke and thereby may contribute to development of cellular senescence.

Results

Primary human bronchial epithelial cells from five healthy donors were cultured, treated with or without 1.5% cigarette smoke extract (CSE) for 24 h or were passaged into replicative senescence. Transcriptome changes were monitored using RNA-seq in CSE and non-CSE exposed cells and those passaged into replicative senescence. We found that, among 1534 genes differentially regulated during senescence and 599 after CSE exposure, 243

SUBMITTER: Voic H 

PROVIDER: S-EPMC6325884 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature