The effect of isolation methods of tomato pollen on the results of metabolic profiling.

ABSTRACT:

Introduction

Untargeted metabolomics is a powerful tool to detect hundreds of metabolites within a given tissue and to compare the metabolite composition of samples in a comprehensive manner. However, with regard to pollen research such comprehensive metabolomics approaches are yet not well developed. To enable isolation of pollen that is tightly enclosed within the anthers of the flower, such as immature pollen, the current pollen isolation protocols require the use of a watery solution. These protocols raise a number of concerns for their suitability in metabolomics analyses, in view of possible metabolic activities in the pollen and contamination with anther metabolites.

Objectives

We assessed the effect of different sample preparation procedures currently used for pollen

SUBMITTER: Paupiere MJ 

PROVIDER: S-EPMC6326007 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature