Integrated pathogen load and dual transcriptome analysis of systemic host-pathogen interactions in severe malaria.

ABSTRACT: The pathogenesis of infectious diseases depends on the interaction of host and pathogen. In Plasmodium falciparum malaria, host and parasite processes can be assessed by dual RNA sequencing of blood from infected patients. We performed dual transcriptome analyses on samples from 46 malaria-infected Gambian children to reveal mechanisms driving the systemic pathophysiology of severe malaria. Integrating these transcriptomic data with estimates of parasite load and detailed clinical information allowed consideration of potentially confounding effects due to differing leukocyte proportions in blood, parasite developmental stage, and whole-body pathogen load. We report hundreds of human and parasite genes differentially expressed between severe and uncomplicated malaria, with distinct p

SUBMITTER: Lee HJ 

PROVIDER: S-EPMC6326353 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature