A Mechanistic Explanation for the Regioselectivity of Nonenzymatic RNA Primer Extension.

ABSTRACT: A working model of nonenzymatic RNA primer extension could illuminate how prebiotic chemistry transitioned to biology. All currently known experimental reconstructions of nonenzymatic RNA primer extension yield a mixture of 2'-5' and 3'-5' internucleotide linkages. Although long seen as a major problem, the causes of the poor regioselectivity of the reaction are unknown. We used a combination of different leaving groups, nucleobases, and templating sequences to uncover the factors that yield selective formation of 3'-5' internucleotide linkages. We found that fast and high yielding reactions selectively form 3'-5' linkages. Additionally, in all cases with high 3'-5' regioselectivity, Watson-Crick base pairing between the RNA monomers and the template is observed at the extension site and a

SUBMITTER: Giurgiu C 

PROVIDER: S-EPMC6326530 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature