A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast.

ABSTRACT: The robust and precise on and off switching of one or more genes of interest, followed by expression or repression is essential for many biological circuits as well as for industrial applications. However, many regulated systems published to date influence the viability of the host cell, show high basal expression or enable only the overexpression of the target gene without the possibility of fine regulation. Herein, we describe an AND gate designed to overcome these limitations by combining the advantages of three well established systems, namely the scaffold RNA CRISPR/dCas9 platform that is controlled by Gal10 as a natural and by LexA-ER-AD as heterologous transcription factor. We hence developed a predictable and modular, versatile expression control system. The selection of a reporter

SUBMITTER: Hofmann A 

PROVIDER: S-EPMC6326796 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature