Skeletal phenotype of the neuropeptide Y knockout mouse.

ABSTRACT: Neuropeptide Y (NPY) is involved in multiple processes such as behavior, energy and bone metabolism. Previous studies have relied on global NPY depletion to examine its effects on bone. However, this approach is unable to distinguish the central or local source of NPY influencing bone. Our aim was to identify which cells within the skeleton express Npy and establish a model that will enable us to differentiate effects of NPY derived from different cell types. We have generated the NPY floxed (NPY^flox) mice using CRISPR technology. By crossing the NPY^flox mice with Hypoxanthine Phosphoribosyltransferase 1 (Hprt)-cre to generate a global knockout, we were able to validate and confirm loss of Npy transcript and protein in our global NPYKO. Global deletion of NPY results

SUBMITTER: Wee NKY 

PROVIDER: S-EPMC6326877 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature