Functionalized Magnetic Bacterial Cellulose Beads as Carrier for Lecitase® Ultra Immobilization.

ABSTRACT: Bacterial cellulose spheres subjected to amination and inlaid modification with superparamagnetic molecules were analyzed with regard to possibility of their application as an immobilization carrier of Lecitase® Ultra (LU) enzyme. The starting point to obtain the carrier was synthesis of bacterial cellulose spheres performed in shaking cultures of Komagataeibacter xylinus. These spheres were subsequently subjected to a multi-stage modification to increase the efficiency of the immobilization process and to separate product from the reaction medium. Maximal yield of Lecitase® Ultra immobilization equaled 70%. It was also found that immobilization process did not affect the pH and LU temperature optimum. Moreover, immobilized enzyme exhibited similar temperature stability profile as its nati

SUBMITTER: Drozd R 

PROVIDER: S-EPMC6326999 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature