Automated, high-throughput measurement of size and growth curves of small organisms in well plates.

ABSTRACT: Organism size and growth curves are important biological characteristics. Current methods to measure organism size, and in particular growth curves, are often resource intensive because they involve many manual steps. Here we demonstrate a method for automated, high-throughput measurements of size and growth in individual aquatic invertebrates kept in microtiter well-plates. We use a spheroid counter (Cell³iMager, cc-5000) to automatically measure size of seven different freshwater invertebrate species. Further, we generated calibration curves (linear regressions, all p < 0.0001, r² >=0.9 for Ceriodaphnoa dubia, Asellus aquaticus, Daphnia magna, Daphnia pulex; r² >=0.8 for Hyalella azteca, Chironomus spec. larvae and Culex spec. larvae) to convert size meas

SUBMITTER: Duckworth J 

PROVIDER: S-EPMC6327043 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature