Gain of CTCF-Anchored Chromatin Loops Marks the Exit from Naive Pluripotency.

ABSTRACT: The genome of pluripotent stem cells adopts a unique three-dimensional architecture featuring weakly condensed heterochromatin and large nucleosome-free regions. Yet, it is unknown whether structural loops and contact domains display characteristics that distinguish embryonic stem cells (ESCs) from differentiated cell types. We used genome-wide chromosome conformation capture and super-resolution imaging to determine nuclear organization in mouse ESC and neural stem cell (NSC) derivatives. We found that loss of pluripotency is accompanied by widespread gain of structural loops. This general architectural change correlates with enhanced binding of CTCF and cohesins and more pronounced insulation of contacts across chromatin boundaries in lineage-committed cells. Reprogramming NSCs to plurip

SUBMITTER: Pekowska A 

PROVIDER: S-EPMC6327227 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature