An auto-inducible phosphate-controlled expression system of Bacillus licheniformis.

ABSTRACT:

Background

A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterologous proteins at low cost. A novel phosphate-regulated expression system was constructed using the promoter of the phytase encoding gene phyL from Bacillus licheniformis for the overexpression of proteins in this industrially relevant host.

Results

It is shown that the phyL promoter enables a strong overexpression of the heterologous genes amyE and xynA in B. licheniformis when cells were subjected to phosphate limitation. Whether B. licheniformis can use phytate as an alternative phosphate source and how this substrate influences the PphyL controlled gene e

SUBMITTER: Trung NT 

PROVIDER: S-EPMC6327384 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature