Detection of endoplasmic reticulum stress and the unfolded protein response in naturally-occurring endocrinopathic equine laminitis.

ABSTRACT:

Background

Laminitis is often associated with endocrinopathies that cause hyperinsulinemia and is also induced experimentally by hyperinsulinemia, suggesting that insulin initiates laminitis pathogenesis. Hyperinsulinemia is expected to activate pro-growth and anabolic signaling pathways. We hypothesize that chronic over-stimulation of these pathways in lamellar tissue results in endoplasmic reticulum stress, contributing to tissue pathology, as it does in human metabolic diseases. We tested this hypothesis by asking whether lamellar tissue from horses with naturally-occurring endocrinopathic laminitis showed expression of protein markers of endoplasmic reticulum stress.

Results

Three markers of endoplasmic reticulum stress, spliced XBP1, Grp78/BiP and Grp94, were upregulate

SUBMITTER: Cassimeris L 

PROVIDER: S-EPMC6327420 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature