Longitudinal genome-wide DNA methylation analysis uncovers persistent early-life DNA methylation changes.

ABSTRACT:

Background

Early life is a period of drastic epigenetic remodeling in which the epigenome is especially sensitive to extrinsic and intrinsic influence. However, the epigenome-wide dynamics of the DNA methylation changes that occur during this period have not been sufficiently characterized in longitudinal studies.

Methods

To this end, we studied the DNA methylation status of more than 750,000 CpG sites using Illumina MethylationEPIC arrays on 33 paired blood samples from 11 subjects at birth and at 5 and 10 years of age, then characterized the chromatin context associated with these loci by integrating our data with histone, chromatin-state and enhancer-element external datasets, and, finally, validated our results through bisulfite pyrosequencing in two independent longitud

SUBMITTER: Perez RF 

PROVIDER: S-EPMC6327427 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature