Transcriptional profiling of Pseudomonas aeruginosa and Staphylococcus aureus during in vitro co-culture.

ABSTRACT:

Background

Co-colonization by Pseudomonas aeruginosa and Staphylococcus aureus is frequent in cystic fibrosis patients. Polymicrobial infections involve both detrimental and beneficial interactions between different bacterial species. Such interactions potentially indirectly impact the human host through virulence, antibiosis and immunomodulation.

Results

Here we explored the responses triggered by the encounter of these two pathogens to identify early processes that are important for survival when facing a potential competitor. Transcriptional profiles of both bacteria were obtained after 3 h co-culture and compared to the respective mono-culture using RNAseq. Global responses in both bacteria included competition for nitrogen sources, amino acids and increased tRNA levels.

SUBMITTER: Tognon M 

PROVIDER: S-EPMC6327441 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature