A cytosolic NAD⁺-dependent GPDH from maize (ZmGPDH1) is involved in conferring salt and osmotic stress tolerance.

ABSTRACT:

Background

Plant glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the reduction of dihydroxyacetone phosphate (DHAP) to produce glycerol-3-phosphate (G-3-P), and plays a key role in glycerolipid metabolism as well as stress responses.

Results

In this study, we report the cloning, enzymatic and physiological characterization of a cytosolic NAD⁺-dependent GPDH from maize. The prokaryotic expression of ZmGPDH1 in E.coli showed that the enzyme encoded by ZmGPDH1 was capable of catalyzing the reduction of DHAP in the presence of NADH. The functional complementation analysis revealed that ZmGPDH1 was able to restore the production of glycerol-3-phosphate and glycerol in AtGPDHc-deficient mutants. Furthermore, overexpression of ZmGPDH1 remarkably enhanced the toleranc

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC6327487 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature