Improvement of isoprene production in Escherichia coli by rational optimization of RBSs and key enzymes screening.

ABSTRACT:

Background

As an essential platform chemical mostly used for rubber synthesis, isoprene is produced in industry through chemical methods, derived from petroleum. As an alternative, bio-production of isoprene has attracted much attention in recent years. Previous researches were mostly focused on key enzymes to improve isoprene production. In this research, besides screening of key enzymes, we also paid attention to expression intensity of non-key enzymes.

Results

Firstly, screening of key enzymes, IDI, MK and IspS, from other organisms and then RBS optimization of the key enzymes were carried out. The strain utilized IDI_sa was firstly detected to produce more isoprene than other IDIs. IDI_sa expression was improved after RBS modification, leading to 1610

SUBMITTER: Li M 

PROVIDER: S-EPMC6327615 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature