In vitro wound healing of tumor cells: inhibition of cell migration by selected cytotoxic alkaloids.

ABSTRACT:

Background

Cell migration is involved in several pathological processes such as tumor invasion, neoangiogenesis and metastasis. Microtubules are needed in directional migration.

Methods

To investigate the effects of microtubule-binding agents (paclitaxel, vinblastine, colchicine, podophyllotoxin), benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelidonine) and other anti-tumor drugs (homoharringtonine, doxorubicin) on cell migration, we performed the in vitro wound healing assay. The interactions between selected alkaloids and microtubules were studied via U2OS cells expressing microtubule-GFP markers.

Results

The microtubule-binding natural products paclitaxel, vinblastine, colchicine and podophyllotoxin significantly altered microtubule dynamics in livi

SUBMITTER: Wang X 

PROVIDER: S-EPMC6327619 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature