Extended live-tracking and quantitative characterization of wound healing and cell migration with SiR-Hoechst.

ABSTRACT: Cell migration is essential to many life processes, including immune response, tissue repair, and cancer progression. A reliable quantitative characterization of the cell migration can therefore aid in the high throughput screening of drug efficacy in wound healing and cancer treatments. In this work, we report what we believe is the first use of SiR-Hoechst for extended live tracking and automated analysis of cell migration and wound healing. We showed through rigorous statistical comparisons that this far-red label does not affect migratory behavior. We observed excellent automated tracking of random cell migration, in which the motility parameters (speed, displacement, path length, directionality ratio, persistence time, and direction autocorrelation) obtained closely match those obtain

SUBMITTER: Chung HH 

PROVIDER: S-EPMC6327846 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature