In situ graphene liquid cell-transmission electron microscopy study of insulin secretion in pancreatic islet cells.

ABSTRACT:

Background

Islet cell transplantation is one of the key treatments for type 1 diabetes. Understanding the mechanisms of insulin fusion and exocytosis are of utmost importance for the improvement of the current islet cell transplantation and treatment of diabetes. These phenomena have not been fully evaluated due either to the lack of proper dynamic imaging, or the lack of proper cell preservation during imaging at nanoscales.

Methods

By maintaining the native environment of pancreatic β-cells between two graphene monolayer sheets, we were able to monitor the subcellular events using in situ graphene liquid cell (GLC)-transmission electron microscopy (TEM) with both high temporal and high spatial resolution.

Results

For the first time, the nucleation and growth of insu

SUBMITTER: Firlar E 

PROVIDER: S-EPMC6327893 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature