I-SceI Meganuclease-mediated transgenesis in the acorn worm, Saccoglossus kowalevskii.

ABSTRACT: Hemichordates are a phylum of marine invertebrate deuterostomes that are closely related to chordates, and represent one of the most promising models to provide insights into early deuterostome evolution. The genome of the hemichordate, Saccoglossus kowalevskii, reveals an extensive set of non-coding elements conserved across all three deuterostome phyla. Functional characterization and cross-phyla comparisons of these putative regulatory elements will enable a better understanding of enhancer evolution, and subsequently how changes in gene regulation give rise to morphological innovation. Here, we describe an efficient method of transgenesis for the characterization of non-coding elements in S. kowalevskii. We first test the capacity of an I-SceI transgenesis system to drive ubiquitous or

SUBMITTER: Minor PJ 

PROVIDER: S-EPMC6327965 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature