A standardized immune phenotyping and automated data analysis platform for multicenter biomarker studies.

ABSTRACT: The analysis and validation of flow cytometry-based biomarkers in clinical studies are limited by the lack of standardized protocols that are reproducible across multiple centers and suitable for use with either unfractionated blood or cryopreserved PBMCs. Here we report the development of a platform that standardizes a set of flow cytometry panels across multiple centers, with high reproducibility in blood or PBMCs from either healthy subjects or patients 100 days after hematopoietic stem cell transplantation. Inter-center comparisons of replicate samples showed low variation, with interindividual variation exceeding inter-center variation for most populations (coefficients of variability <20% and interclass correlation coefficients >0.75). Exceptions included low-abundance populations de

SUBMITTER: Ivison S 

PROVIDER: S-EPMC6328091 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature