Analysis of antifungal resistance genes in Candida albicans and Candida glabrata using next generation sequencing.

ABSTRACT:

Introduction/objectives

An increase in antifungal resistant Candida strains has been reported in recent years. The aim of this study was to detect mutations in resistance genes of azole-resistant, echinocandin-resistant or multi-resistant strains using next generation sequencing technology, which allows the analysis of multiple resistance mechanisms in a high throughput setting.

Methods

Forty clinical Candida isolates (16 C. albicans and 24 C. glabrata strains) with MICs for azoles and echinocandins above the clinical EUCAST breakpoint were examined. The genes ERG11, ERG3, TAC1 and GSC1 (FKS1) in C. albicans, as well as ERG11, CgPDR1, FKS1 and FKS2 in C. glabrata were sequenced.

Results

Fifty-four different missense mutations were identified, 13 of which have not been

SUBMITTER: Spettel K 

PROVIDER: S-EPMC6328131 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature