Designing antibody against highly conserved region of dengue envelope protein by in silico screening of scFv mutant library.

ABSTRACT: Dengue being one of the deadliest diseases of tropical regions, enforces to put continuous efforts for the development of vaccine and effective therapeutics. Most of the antibodies generated during dengue infection are non-neutralizing and cause antibody dependent enhancement. Hence, making a potent neutralizing antibody against all four dengue serotypes could be very effective for the treatment. However, designing a single antibody for all serotypes is difficult due to variation in protein sequences. Therefore, the objective is to identify conserved region of dengue envelope protein and then develop an antibody against that conserved region. Before advancing to the development of such an antibody, it is desirable to validate the interactions between antibody and dengue envelope protein. I

SUBMITTER: Rathore AS 

PROVIDER: S-EPMC6328183 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature