An Improved Method for Protein Extraction from Minuscule Quantities of Fungal Biomass.

ABSTRACT: Filamentous fungi are ubiquitous eukaryotes having chitin as a major constituent of the cell wall. Chitin is tough to lyse due to which the intracellular fungal proteins are not readily accessible. The problem is further enhanced when the biomass to be analyzed for protein studies is too little due to the extreme experimental parameters under consideration such as increased or lowered pH, temperature, hydrostatic pressure, nutrients, etc. The method described here is capable of obtaining proteins from minuscule quantities of biomass (~5 mg lyophilized biomass). In this study, different lysing conditions and varied composition of extraction buffers were tried to obtain maximum protein of high quality. Lysis with zirconium beads in a combination buffer system (Tris-MgCl₂ buffer, u

SUBMITTER: Krishnaswamy A 

PROVIDER: S-EPMC6328415 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature