Cloning, Expression and Characterization of Two Beta Carbonic Anhydrases from a Newly Isolated CO₂ Fixer, Serratia marcescens Wy064.

ABSTRACT: Bacterial strains from karst landform soil were enriched via chemostat culture in the presence of sodium bicarbonate. Two chemolithotrophic strains were isolated and identified as Serratia marcescens Wy064 and Bacillus sp. Wy065. Both strains could grow using sodium bicarbonate as the sole carbon source. Furthermore, the supplement of the medium with three electron donors (Na₂S, NaNO₂, and Na₂S₂O₃) improved the growth of both strains. The activities of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) could be detected in the crude enzyme of strain Wy064, implying that the strain Wy064 might employ Calvin cycle to fix CO₂. S. marcescens genome mining revealed four pote

SUBMITTER: Chen F 

PROVIDER: S-EPMC6328416 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature