Hexokinase 2 as a novel selective metabolic target for rheumatoid arthritis.

ABSTRACT:

Objectives

Recent studies indicate that glucose metabolism is altered in rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS). Hexokinases (HKs) catalyse the first step in glucose metabolism, and HK2 constitutes the principal HK inducible isoform. We hypothesise that HK2 contributes to the synovial lining hypertrophy and plays a critical role in bone and cartilage damage.

Methods

HK1 and HK2 expression were determined in RA and osteoarthritis (OA) synovial tissue by immunohistochemistry. RA FLS were transfected with either HK1 or HK2 siRNA, or infected with either adenovirus (ad)-GFP, ad-HK1 or ad-HK2. FLS migration and invasion were assessed. To study the role of HK2 in vivo, 10⁸ particles of ad-HK2 or ad-GFP were injected into the knee of wild-type mice.

SUBMITTER: Bustamante MF 

PROVIDER: S-EPMC6328432 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature