Novel circular RNA expression profile of uveal melanoma revealed by microarray.

ABSTRACT:

Objective

The present study aimed to investigate circular RNA (circRNA) expression in uveal melanoma (UM).

Methods

First, we used microarray to compare the expression profiles of circRNA in five UM samples and five normal uvea tissues. Next, bioinformatics analyses, including gene ontology (GO) analysis and pathway analysis, were applied to study these differentially expressed circRNAs to predict pathogenic pathways that may be involved. Quantitative real-time polymerase chain reaction (qRT-PCR) in 20 UM samples and 20 normal uvea samples was used to confirm the circRNA expression profiles obtained from the microarray data. Finally, we analyzed the interaction between validated circRNAs and their potential cancer-associated miRNA targets.

Results

In total, 50,579 circ

SUBMITTER: Yang X 

PROVIDER: S-EPMC6328506 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature