Ca²⁺-activated Cl^- channel TMEM16A/ANO1 identified in zebrafish skeletal muscle is crucial for action potential acceleration.

ABSTRACT: The Ca²⁺-activated Cl^- channel (CaCC) TMEM16A/Anoctamin 1 (ANO1) is expressed in gastrointestinal epithelia and smooth muscle cells where it mediates secretion and intestinal motility. However, ANO1 Cl^- conductance has never been reported to play a role in skeletal muscle. Here we show that ANO1 is robustly expressed in the highly evolved skeletal musculature of the euteleost species zebrafish. We characterised ANO1 as bonafide CaCC which is activated close to maximum by Ca²⁺ ions released from the SR during excitation-contraction (EC) coupling. Consequently, our study addressed the question about the physiological advantage of implementation of ANO1 into the euteleost skeletal-muscle EC coupling machinery. Our results reveal that Cl^-

SUBMITTER: Dayal A 

PROVIDER: S-EPMC6328546 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature