Extractive Fermentation for Process integration and amplified pullulan production by A. pullulans in Aqueous Two Phase Systems.

ABSTRACT: Extractive fermentation technique or in situ product recovery process is a novel technique to segregate the desired product simultaneously in a fermentation process. For economic and high yield production of pullulan, Extractive fermentation process was applied fermentation process of A. pullulans. Aqueous Two Phase system (ATPS) systems were designed with various molecular mass of PEG (400, 600, 4000 and 6000) and dextran or mono/bi-sodium phosphate salts. Systems with short Tie Line length (TLL) 6.7 and 7.5% w/w for PEG-Salt and PEG-dextran respectively were chosen. Volume ratio for all the systems was kept constant at 1.0 and pH 7.0 for PEG-dextran and PEG-NaH₂PO₄ was maintained, whereas pH 9.0 was kept for PEG-Na₂HPO₄. A. pullulans, was found

SUBMITTER: Badhwar P 

PROVIDER: S-EPMC6328562 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature