µLAS: Sizing of expanded trinucleotide repeats with femtomolar sensitivity in less than 5 minutes.

ABSTRACT: We present µLAS, a lab-on-chip system that concentrates, separates, and detects DNA fragments in a single module. µLAS speeds up DNA size analysis in minutes using femtomolar amounts of amplified DNA. Here we tested the relevance of µLAS for sizing expanded trinucleotide repeats, which cause over 20 different neurological and neuromuscular disorders. Because the length of trinucleotide repeats correlates with the severity of the diseases, it is crucial to be able to size repeat tract length accurately and efficiently. Expanded trinucleotide repeats are however genetically unstable and difficult to amplify. Thus, the amount of amplified material to work with is often limited, making its analysis labor-intensive. We report the detection of heterogeneous allele lengths in 8 samples from myoto

SUBMITTER: Malbec R 

PROVIDER: S-EPMC6328573 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature