Novel Endotype Xanthanase from Xanthan-Degrading Microbacterium sp. Strain XT11.

ABSTRACT: Under general aqueous conditions, xanthan appears in an ordered conformation, which makes its backbone largely resistant to degradation by known cellulases. Therefore, the xanthan degradation mechanism is still unclear because of the lack of an efficient hydrolase. Here, we report the catalytic properties of MiXen, a xanthan-degrading enzyme identified from the genus Microbacterium MiXen is a 952-amino-acid protein that is unique to strain XT11. Both the sequence and structural features suggested that MiXen belongs to a new branch of the GH9 family and has a multimodular structure in which a catalytic (α/α)₆ barrel is flanked by an N-terminal Ig-like domain and by a C-terminal domain that has very few homologues in sequence databases and functions as a carbohydrate-bindin

SUBMITTER: Yang F 

PROVIDER: S-EPMC6328773 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature