Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis.

ABSTRACT:

Background

Although mesenchymal stem/stromal cell (MSC) chondrogenic differentiation has been thoroughly investigated, the rudiments for enhancing chondrogenesis have remained largely dependent on external cues. Focus to date has been on extrinsic variables such as soluble signals, culture conditions (bioreactors), and mechanical stimulation. However, the role of intrinsic mechanisms of MSC programming-based mechanobiology remains to be explored. Since aggregation of MSCs, a prerequisite for chondrogenesis, generates tension within the cell agglomerate, we inquired if the initial number of cells forming the aggregate (aggregate cell number (ACN)) can impact chondrogenesis.

Methods

Aggregates of varying ACN were formed using well-established centrifugation approach. Progressi

SUBMITTER: Sarem M 

PROVIDER: S-EPMC6329065 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature