Translational control mechanisms in cutaneous malignant melanoma: the role of eIF2α.

ABSTRACT:

Background

Melanoma cells develop adaptive responses in order to cope with particular conditions of tumor microenvironment, characterized by stress conditions and deregulated proliferation. Recently, the interplay between the stress response and the gene expression programs leading to metastatic spread has been reported.

Methods

We evaluated levels and localization of eIF2α/peIF2α in V600BRAF and wtBRAF metastatic melanoma cell lines by means of western blot and confocal microscopy analyses. Furthermore, we performed a sequence analyses and structure and dynamics studies of eIF2α protein to reveal the role of eIF2α and its correlations in different pathways involved in the invasive phase of melanoma.

Results

We found peIF2α both in cytoplasm and nucleus. Nuclear local

SUBMITTER: Maida I 

PROVIDER: S-EPMC6329103 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature