Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content.

ABSTRACT:

Background

Rapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary.

Results

Here, proteomic and genomic approaches were used to analyze the germination process in B. napus seeds with different oil content. The identification of 165 differentially expressed proteins (DEPs) in the germinating seeds of B. napus with high and low oil content was accomplished by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE). The comparative proteomic results revealed

SUBMITTER: Gu J 

PROVIDER: S-EPMC6329107 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature