Modelling overflow metabolism in Escherichia coli with flux balance analysis incorporating differential proteomic efficiencies of energy pathways.

ABSTRACT:

Background

The formation of acetate by fast-growing Escherichia coli (E. coli) is a commonly observed phenomenon, often referred to as overflow metabolism. Among various studies that have been carried over decades, a recent work (Basan, M. et al. Nature 528, 99-104, 2015) suggested and validated that it is the differential proteomic efficiencies in energy biogenesis between fermentation and respiration that lead to the production of acetate at rapid growth conditions, as the consequence of optimally allocating the limited proteomic resource. In the current work, we attempt to incorporate this newly developed proteome allocation theory into flux balance analysis (FBA) to capture quantitatively the extent of overflow metabolism in different E. coli strains.

Results

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SUBMITTER: Zeng H 

PROVIDER: S-EPMC6329140 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature