Heme oxygenase 1 facilitates cell proliferation via the B-Raf-ERK signaling pathway in melanoma.

ABSTRACT:

Background

Despite therapeutic advancements (e.g. B-RAF inhibitors) targeting cutaneous melanoma, many cellular processes, including inducible heme oxygenase 1 (HO-1), counteract treatments for malignancies. So there is an urgent need to find biological treatment targets, develop new therapeutic approaches and achieve longer responses. This study aimed to explore the relationship of HO-1 and B-Raf via mediating ERK1/2 signaling on cell cycle in melanoma.

Methods

Immunohistochemistry was applied to evaluate the levels of HO-1 and B-Raf expression in melanoma tissues and adjacent healthy tissues. Co-immunoprecipitation (Co-IP) assessed the interaction of HO-1 with B-Raf. Further study overexpression and knock-down of HO-1 in A375 cell lines, especially knockout HO-1 using CRIS

SUBMITTER: Liu L 

PROVIDER: S-EPMC6329143 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature