Simultaneous rapid detection of Hantaan virus and Seoul virus using RT-LAMP in rats.

ABSTRACT:

Background

Hemorrhagic fever with renal syndrome is in most cases caused by the Hantaan virus (HTNV) and Seoul virus (SEOV). To develop and apply reverse transcription loop-mediated isothermal amplification (RT-LAMP) to detect HTNV and SEOV simultaneously, which was faster, more cost effective, and easier to perform as the target gene amplified rapidly. In this article an assay based on LAMP is demonstrated, which only employs such apparatus as a water bath or a heat block.

Methods

A chromogenic method using the calcein/Mn²⁺ complex and real-time turbidity monitoring method were used to assess reaction progress of the reaction, and the specificity of the RT-LAMP-based assay was assessed by detecting cDNAs/cRNAs generated from Coxsackievirus A16, Influenza virus, l

SUBMITTER: Sui X 

PROVIDER: S-EPMC6329334 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature