hdac4 mediates perichondral ossification and pharyngeal skeleton development in the zebrafish.

ABSTRACT:

Background

Histone deacetylases (HDACs) are epigenetic factors that function to repress gene transcription by removing acetyl groups from the N-terminal of histone lysines. Histone deacetylase 4 (HDAC4), a class IIa HDAC, has previously been shown to regulate the process of endochondral ossification in mice via repression of Myocyte enhancer factor 2c (MEF2C), a transcriptional activator of Runx2, which in turn promotes chondrocyte maturation and production of bone by osteoblasts.

Methods & materials

In this study, we generated two zebrafish lines with mutations in hdac4 using CRISPR/Cas9 and analyzed mutants for skeletal phenotypes and expression of genes known to be affected by Hdac4 expression.

Results

Lines have insertions causing a frameshift in a p

SUBMITTER: DeLaurier A 

PROVIDER: S-EPMC6329341 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature