Overexpression of Notch3 is associated with metastasis and poor prognosis in osteosarcoma patients.

ABSTRACT:

Background

Notch signaling abnormalities are associated with the development of various tumors, including hematopoietic and epithelium-derived tumors. However, the role of Notch signaling in tumors originating from mesenchymal cells is unclear. The effect of Notch3 expression on the prognosis of osteosarcoma and its role and mechanism in osteosarcoma cells have never been reported.

Materials and methods

In this study, we performed a clinicopathological analysis of 70 cases of osteosarcoma, with primary focus on survival. Osteosarcoma cell lines MTH and U2OS were used. After knockdown of Notch3 by lentiviral transfection and siRNA, the cell cycle, cell viability, and wound healing capacity were assessed. Subsequently, the Transwell assay was performed, and the expression leve

SUBMITTER: Tang XF 

PROVIDER: S-EPMC6329347 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature