MiR-302 cluster inhibits angiogenesis and growth of K562 leukemia cells by targeting VEGFA.

ABSTRACT:

Background

miR-302 cluster has been reported as a tumor suppressor in many human cancers; yet, its function in chronic myeloid leukemia (CML) tumorigenesis remains largely unclear. The study was aimed to explore the functional roles of miR-302 cluster in CML progression.

Materials and methods

Quantitative reverse transcriptase PCR and Western blot were performed to evaluate miR-302 cluster and vascular endothelial growth factor A (VEGFA) expression levels. Cell Counting Kit-8 assay, colony formation assay and human umbilical vein endothelial cell line capillary tube formation were used to determine the influence of miR-302 cluster on the growth and angiogenesis of K562 cells, respectively. Luciferase reporter assay was employed to confirm the direct target interaction betwee

SUBMITTER: Cao J 

PROVIDER: S-EPMC6329480 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature