Improved DNA extraction technique from clot for the diagnosis of Chagas disease.

ABSTRACT:

Background

The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater sensitivity than either buffy coat or whole blood samples. However, phenol-chloroform DNA extraction from clot samples is difficult and toxic. The objective of the present study was to improve and develop a more sensitive method to recover parasite DNA from clot samples for the diagnosis of Chagas disease.

Methodology/principal findings

A total of 265 match pair samples of whole blood-guanidine (GEB) and clot samples were analyzed; 150 were from Chagas seropositive subjects. DNA was extracted from both whole blood-guanidine samples, using a previously standa

SUBMITTER: Mayta H 

PROVIDER: S-EPMC6329489 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature