The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling.

ABSTRACT: Complex interplay between cardiac tissues is crucial for their integrity. The flow responsive transcription factor KLF2, which is expressed in the endocardium, is vital for cardiovascular development but its exact role remains to be defined. To this end, we mutated both klf2 paralogues in zebrafish, and while single mutants exhibit no obvious phenotype, double mutants display a novel phenotype of cardiomyocyte extrusion towards the abluminal side. This extrusion requires cardiac contractility and correlates with the mislocalization of N-cadherin from the lateral to the apical side of cardiomyocytes. Transgenic rescue data show that klf2 expression in endothelium, but not myocardium, prevents this cardiomyocyte extrusion phenotype. Transcriptome analysis of klf2 mutant

SUBMITTER: Rasouli SJ 

PROVIDER: S-EPMC6329608 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature