SPECT/CT Imaging of Mycobacterium tuberculosis Infection with [¹²⁵I]anti-C3d mAb.

ABSTRACT:

Purpose

Diagnosis and therapeutic monitoring of chronic bacterial infection requires methods to detect and localize sites of infection accurately. Complement C3 activation fragments are generated and covalently bound to selective bacterial pathogens during the immune response and can serve as biomarkers of ongoing bacterial infection. We have developed several probes for detecting tissue-bound C3 deposits, including a monoclonal antibody (mAb 3d29) that recognizes the tissue-bound terminal processing fragments iC3b and C3d but does not recognize native circulating C3 or tissue-bound C3b.

Procedures

To determine whether mAb 3d29 could be used to detect chronic Mycobacterium tuberculosis infection non-invasively, aerosol-infected female C3HeB/FeJ mice were injected with [

SUBMITTER: Foss CA 

PROVIDER: S-EPMC6329677 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature