High resolution fingerprinting of single and double-stranded RNA using ion-pair reverse-phase chromatography.

ABSTRACT: The emergence of new sustainable approaches for insect management using RNA interference (RNAi) based insecticides has created the demand for high throughput analytical techniques to fully characterise and accurately quantify double stranded RNA (dsRNA) prior to downstream RNAi applications. In this study we have developed a method for the rapid characterisation of single stranded and double stranded RNA using high resolution RNase mapping in conjunction with ion-pair reverse-phase chromatography utilising a column with superficially porous particles. The high resolution oligoribonucleotide map provides an important 'fingerprint' for identity testing and bioprocess monitoring. Reproducible RNA mapping chromatograms were generated from replicate analyses. Moreover, this approach was used to

SUBMITTER: Nwokeoji AO 

PROVIDER: S-EPMC6329874 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature