Plasmodium vivax readiness to transmit: implication for malaria eradication.

ABSTRACT:

Background

The lack of a continuous long-term in vitro culture system for Plasmodium vivax severely limits our knowledge of pathophysiology of the most widespread malaria parasite. To gain direct understanding of P. vivax human infections, we used Next Generation Sequencing data mining to unravel parasite in vivo expression profiles for P. vivax, and P. falciparum as comparison.

Results

We performed cloud and local computing to extract parasite transcriptomes from publicly available raw data of human blood samples. We developed a Poisson Modelling (PM) method to confidently identify parasite derived transcripts in mixed RNAseq signals of infected host tissues. We successfully retrieved and reconstructed parasite transcriptomes from infected patient blood as early as the firs

SUBMITTER: Adapa SR 

PROVIDER: S-EPMC6330404 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature