MicroRNA-regulated pathways of flow-stimulated angiogenesis and vascular remodeling in vivo.

ABSTRACT:

Background

Vascular shear stress promotes endothelial cell sprouting in vitro. The impact of hemodynamic forces on microRNA (miRNA) and gene expression within growing vascular networks in vivo, however, remain poorly investigated. Arteriovenous (AV) shunts are an established model for induction of neoangiogenesis in vivo and can serve as a tool for analysis of hemodynamic effects on miRNA and gene expression profiles over time.

Methods

AV shunts were microsurgically created in rats and explanted on postoperative days 5, 10 and 15. Neoangiogenesis was confirmed by histologic analysis and micro-computed tomography. MiRNA and gene expression profiles were determined in tissue specimens from AV shunts by microarray analysis and quantitative real-time polymerase chain reaction an

SUBMITTER: Henn D 

PROVIDER: S-EPMC6330440 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature