Single cell sequencing reveals gene expression signatures associated with bone marrow stromal cell subpopulations and time in culture.

ABSTRACT:

Background

Bone marrow stromal cells (BMSCs) are a heterogeneous population that participates in wound healing, immune modulation and tissue regeneration. Next generation sequencing was used to analyze transcripts from single BMSCs in order to better characterize BMSC subpopulations.

Methods

Cryopreserved passage 2 BMSCs from one healthy subject were cultured through passage 10. The transcriptomes of bulk BMSCs from designated passages were analyzed with microarrays and RNA sequencing (RNA-Seq). For some passages, single BMSCs were separated using microfluidics and their transcriptomes were analyzed by RNA-Seq.

Results

Transcriptome analysis by microarray and RNA-Seq of unseparated BMSCs from passages 2, 4, 6, 8, 9 and 10 yielded similar results; both data sets groupe

SUBMITTER: Liu S 

PROVIDER: S-EPMC6330466 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature