Arrayed functional genetic screenings in pluripotency reprogramming and differentiation.

ABSTRACT: Thoroughly understanding the molecular mechanisms responsible for the biological properties of pluripotent stem cells, as well as for the processes involved in reprograming, differentiation, and transition between Naïve and Primed pluripotent states, is of great interest in basic and applied research. Although pluripotent cells have been extensively characterized in terms of their transcriptome and miRNome, a comprehensive understanding of how these gene products specifically impact their biology, depends on gain- or loss-of-function experimental approaches capable to systematically interrogate their function. We review all studies carried up to date that used arrayed screening approaches to explore the function of these genetic elements on those biological contexts, using focused or genom

SUBMITTER: Panepucci RA 

PROVIDER: S-EPMC6330485 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature