IFNγ and TNFα synergistically induce apoptosis of mesenchymal stem/stromal cells via the induction of nitric oxide.

ABSTRACT:

Background

Mesenchymal stem/stromal cells (MSCs) have been widely used to treat various inflammatory diseases. The immunomodulatory capabilities of MSCs are usually licensed by inflammatory cytokines and may vary depending on the levels and the types of inflammatory cytokines. However, how the inflammatory microenvironment affects the fate of MSCs remains elusive. Here we characterized the molecular mechanism underlying the apoptosis of mouse MSCs triggered by the synergistic action of IFNγ and TNFα.

Methods

We isolated and expanded MSCs by flushing the femoral and tibial bone marrow of wild-type, iNOS^-/-, and Fas^-/- mice. BM-MSCs were treated with IFNγ and TNFα in vitro, and cell viability was evaluated by a CCK-8 kit. Apoptosis was assessed by Annexin

SUBMITTER: Li X 

PROVIDER: S-EPMC6330503 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature