Ectopic expression of ORANGE promotes carotenoid accumulation and fruit development in tomato.

ABSTRACT: Carotenoids are critically important to plants and humans. The ORANGE (OR) gene is a key regulator for carotenoid accumulation, but its physiological roles in crops remain elusive. In this study, we generated transgenic tomato ectopically overexpressing the Arabidopsis wild-type OR (AtOR^WT ) and a 'golden SNP'-containing OR (AtOR^His ). We found that AtOR^His initiated chromoplast formation in very young fruit and stimulated carotenoid accumulation at all fruit developmental stages, uncoupled from other ripening activities. The elevated levels of carotenoids in the AtOR lines were distributed in the same subplastidial fractions as in wild-type tomato, indicating an adaptive response of plastids to sequester the increased carotenoids. Mic

SUBMITTER: Yazdani M 

PROVIDER: S-EPMC6330546 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature