Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process.

ABSTRACT:

Background

The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs). However, recent advancement of biotechnology has shown substantial recovery of bioactive protein from such insoluble IBs by solubilization and refolding processes. In addition, gene fusion technology has also widely been used to improve the soluble protein production using E. coli. This study demonstrates that mild-solubilization and in vitro refolding strategies, both are capable to recover soluble scFv protein from bacterial IBs, although the degree of success is greatly influenced by different fusion tags with the target protein.

Results

It was observed that

SUBMITTER: Sarker A 

PROVIDER: S-EPMC6330739 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature